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Figure 1. Organizer-inducing factors activate Xhairy2b expression. A-F: Spatial expression patterns of Xhairy2b by whole-mount in situ hybridization at stage 10.5. Cleared embryos are shown in lateral view and dorsal is to the left. A: Uninjected control embryo. B-F: Ectopic induction of Xhairy2b expression in embryos ventrally microinjected with mRNAs encoding organizer-inducing factors. mRNAs of activin (B), BVg1 (C), Xnr1 (D), Xnr2 (E), and follistatin (F) were injected at the eight-cell stage. Arrowheads show the ectopic expression of Xhairy2b. |
