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Figure 7. Zfp36 mRNA overexpression alters the expression of notch signalling pathway members esr5 and hairy2a. 250 pg of Xenopus zfp36 (a, b, i, j), zfp36l1 (c, d), zfp36l2 (e, f) or mouse zfp36 (mzfp36, g, h, k, l) mRNAs were injected into one blastomere of two-cell stage embryos and developing embryos were fixed at stage 25 (a and k, l) or stage 28 (i, j) and analyzed by in situ hybridization for esr5 and hairy2a expression. Arrows in a, c, e and g mark the presomitic mesoderm (psm) and the pronephros region (pn) in i respectively. Stars in a, c, e and g mark the first two somitomeres. |
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Figure S3. mpaired pronephros morphogenesis caused by zfp36 depletion is largely independent of Notch pathway, proliferation or apoptosis. 8-cell stage embryos were injected unilaterally with 20 ng of zfp36 morpholinos together with 250 pg of lacZ mRNA tracer and analysed at stage 33/34 for Hairy2a expression by whole mount in situ hybridization (a, b), at stage 32 by TUNEL assay (c, d) or at stage 28 by immunohistochemistry with anti-phospho-Histone H3 antibody (Phospho-H3) (e, f). |
