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Fig. 1. X-delta-2 loss of function perturbs somite formation and affects the expression of segmentation genes. Embryos were injected with two different concentrations of X-delta-2 MO (C and D) or with a dominant negative form of X-delta-2, X-delta-2tr (B). Embryos were cultivated until stage 28 and the somites were analysed by whole-mount in situ hybridisation using MyoD as a marker. Embryos are shown from the lateral side with anterior to the left. A higher magnification of the somite area indicated by the dashed square is shown in the lower right corner. Krox-20 expression in the hindbrain and engrailed expression in the midbrain–hindbrain boundary is still present when X-delta-2 is down regulated (B–D). The expression of two segmentation genes ESR-4 (E,F) and Hairy-2a (G,H) is also downregulated in the X-Delta-2 MO injected side (F and H, respectively). Embryos at neurula stage are shown from the dorsal side, anterior up (E,F) and at early tailbud stage from the dorsal side, anterior to the left (G,H). Arrowhead indicates the injected side of the embryo. |
