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Fig. 5. Transcription of dorsal and ventral marker genes in embryos overexpressing BMP-2. Each 1 ng of in vitro transcribed BMP-2 mRNA was injected either into the two dorsal or into the two ventral blastomeres of four-cell stage Xenopus embryos. When uninjected embryos (control) had reached stage 32 to 35, the embryos were fixed and analysed by whole mount in situ hybridization. (A-C): α-Cardiac actin α1 (Stutz and Spohr, 1986); (D-F): class II ß-tubulin (Richter et al., 1988, Good et al., 1989); (G-I): embryonic α-globin (Widmer et al., 1981). Embryos were bleached and cleared in benzylbenzoate/benzyI alcohol (2:l). Arrowheads in (A) or (D) point towards residual α-actin ß-tubulin transcripts, respectively. |
