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FIG. 4. Colocalization of X-msr or B-globin and RLDx. Following in situ hybridization, embryos were embedded in wax and transversely sectioned and then analyzed for the presence of either X-msr or B-globin and RLDx. Black arrowheads in A, D, and G indicate in situ hybridization signal for lateral abdominal veins, dorsal aorta, and ventral blood islands; white arrowheads in B, E, and H indicate the same location viewed under fluorescence. Arrows in C, F, and I show overlay of in situ hybridization signal with fluorescence in same region. (A, B, and C) Examples of X-msr staining in the lateral abdominal veins colocalizing with RLDx from blastomere V2.2. (A) X-msr signal, (B) fluorescence in the same section, and (C) an overlay of both images showing colocalization. (D, E, and F) Examples of X-msr signal colocalizing with RLDx from blastomere V2.1. (D) X-msr staining in the dorsal aorta, which lies ventral to the notochord and hypochord, (E) fluorescence in the same section, and (F) an overlay of both images. (G, H, and I) Examples of B-globin signal in the ventral blood islands colocalizing with RLDx from blastomere D2.1. (G) B-Globin signal, (H) fluorescence in the same section, and (I) an overlay of both images. Dorsal is toward the top of the page. Magnification for A and G is 200x, D is 250x. (J) Schematic indicating planes of section: I (A, B, C), II (D, E, F), and III (G, H, I). Diagram adapted from Nieuwkoop and Faber (1967). da, dorsal aorta; hg, hindgut; lav, lateral abdominal vein; nc, notochord; som, somite; vbi, ventral blood island. |
