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Fig. 7. Effects of Wnt8 and Wnt11 on development of the VBI. Embryos were injected in the marginal zone at the four-cell stage with the indicated amounts of expression vectors encoding xWnt8 or xWnt11 (pCS2-xWnt8 and pCS2-xWnt11), or the indicated amounts of RNA encoding dominant-negative xWnt8 or xWnt11. Embryonic αT3 globin was detected by in situ hybridization at stage 30/35. |
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Fig. 3. Szl negatively regulates development of the ventral blood islands (VBI). (A) Benzidine staining of hemoglobin-containing cells. Embryos were injected with the morpholino oligo MOSZL in both blastomeres at the two-cell stage, or with Szl RNA in the ventral marginal zone at the four-cell stage. (B) In situ hybridization analysis of hematopoietic markers in embryos injected with 20 ng of the morpholino oligos MOSZL or MOC2, or with 40 pg of wSzl RNA in the ventral marginal zone. (a) Expression of xSCL at stage 24. Embryos are shown from the ventral side with anterior upwards. (b) Expression of xGata1 at stage 30. Embryos are shown from the ventral side with anterior downwards. Note that detection of xGata1 in MOC2 injected embryos required a longer reaction time, resulting in darker background staining of head structures. (c) Expression of xSCL at stage 28. (d) Expression of larval globin at stage 33/34. |
