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Fig. 4. Effect of animal pole tissue on a-globin expression in control and cytoplasm-depleted
embryos. (A) Schematic image of the experimental procedure. Pricked and untreated embryos
were allowed to develop until stage 9, and a small (20% of the animal hemisphere) (C,G), middle
(50% of the animal hemisphere) (D,H), or large (100% of the animal hemisphere) (E,I) part of the
animal pole tissue was removed as described in Materials and Methods. Such embryos were further
cultured for 2 days and were used for the whole-mount in situ hybridization analysis to detect the
expression of a-globin. (B-I) Typical examples from each experimental group are shown. Note that
removal of the animal pole tissue caused a drastic reduction in a-globin expression in both control
(B,C,D,E) and cytoplasm-depleted (F,G,H,I) embryos. Scale bar in I indicates 2 mm. |
