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Fig. 2 (Right). Ectopic expression of XRASGRP2 affects vascular formation and induces edema. (A) An uninjected control embryo at stage 43. (B) An embryo in which 1 ng of XRASGRP2 mRNA was injected into the dorsal vegetal blastomeres (DV) at the 8-cell stage. The embryo shows edema. (C-F) Whole-mount in situ hybridization for a hematopoietic marker, globin T3, and an endothelial marker, Xmsr, at stage 31. (C) The expression of globin T3 in an uninjected control embryo (ventral view). (D) Expression of globin T3 in an embryo that was co-injected with XRASGRP2 and β- galactosidase (β-gal) into the ventral vegetal blastomeres (VV) at the 8-cell stage. The expression of globin T3 is abolished at the injection site in the VBI. (E) The expression of Xmsr in an uninjected control embryo (ventral view). (F) The expression of Xmsr in an embryo that was co-injected with XRASGRP2 and β-gal into the VV. Ectopic expression of Xmsr is evident at the injection site in the VBI. Arrowheads indicate Xmsr-positive cells. |
