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igure S5. Regulation of mesodermal gene expression by Pnhd .
For pnhd depletion, each blastomere of four-cell embryos has been injected with 40 ng of pnhd MOsp. The uninjected controls (A, C, E) or pnhd morphants (B, D, F) have been cultured until the indicated stages, fixed and processed for wholemount in situ hybridization with anti-sense probes that are specific for myod (A, B), a-globin (C, D) and chordin (E, F). Number of embryos with the presented phenotype (top) and the total number of embryos per group (bottom) are shown. |
