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Fig. 1. Whole-mount in situ hybridization analysis of Xtie-2
expression in embryos. Lateral views of (A) stage 24 and (B)
stage 27 embryos showing positive signals in the prospective
endocardiac region (h) and in the surrounding regions of the
ventral blood islands (v) in stage 27 embryos. (C) In stage 32
embryos, Xtie-2 is prominently expressed in the endocardium (h)
and in the head mesenchyme around the dorsal region of the
optic capsule (e) and in the mesenchyme overlying the auditory
region (a). The expression is also evident in the branchial region
(b), in the cardinal veins (c) and in the peripheral region of
ventral blood islands (v). (D) Stage 32 embryos hybridized with
Xtie-2 sense probe as a control experiment. Comparison of (E)
Xtie-2 and (F) -globin expression in the ventral mesoderm of a
stage 32 embryo, showing that the expression patterns of these
two genes are mutually exclusive in the ventral blood islands. All
the figures are shown at the same magnification. Bar in (A),
0.5 mm. |