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Figure S8. CST Is Required for Proper Cell Growth of Cardiomyocytes Dorsal to
the Cardiac Ventral Midline
(A) Representative transverse sections of Stage 29 control MO (top) and CstMO
(bottom) injected embryos stained with MHC antibody to mark differentiated
cardiomyocytes, phospho-histone H3 (pH3) antibody to mark cardiac cells in the Mphase
of the cell cycle, and DAPI. Bracket highlights undifferentiated cardiac ventral
midline cells. (B) CST-depleted differentiated cardiomyocytes have an increased mitotic
index at Stage 29. Quantification of the mitotic index was determined by calculating the
percentage of pH3-positive differentiated cardiomyocytes. Bars represent the average
of at least three embryos per condition +/- SEM. *, p<0.01; Scale bars: 200 μm. |
