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Experiment details for greb1

Green YS and Vetter ML (2011) Assay

EBF factors drive expression of multiple classes of target genes governing neuronal development.

Gene Clone Species Stages Anatomy
greb1.L image:5569934 laevis NF stage 15 midbrain-hindbrain boundary , chordal neural plate , neural plate , non-neural ectoderm , anterior neural ridge

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  Figure 3. Downregulation of non-transcription factor candidate target genes after knockdown of EBF2 and EBF3. One cell of two-cell stage embryos was injected with either control MO, both EBF2 MO and EBF3 MO (2MO + 3MO), or dominant negative Xenopus EBF3 (DN-XEBF) mRNA. β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-R) The expression of pcdh8 (B,C), peripherin (E,F), greb1 (H,I), nf-m (K,L), kcnk5 (N,O), and activin beta b (Q,R) is downregulated by EBF2 MO and EBF3 MO, and by DN-XEBF (brackets), while control MO does not change their expression levels (A,D,G,J,M,P). Panels (A-I) and (P-R) are neurula stage embryos (stage 15/16), and (J-O) are tail bud stage embryos (stages 25 to 28). All panels show dorsal views.

Gene Clone Species Stages Anatomy
greb1.L image:5569934 laevis NF stage 15 olfactory placode , neural plate , presumptive paraxial mesoderm
greb1.L image:5569934 laevis NF stage 23 somite , ventral blood island , involuted dorsal mesoderm , mesenchyme , neural tube , [+]
greb1.L image:5569934 laevis NF stage 28 brain , forebrain , midbrain , hindbrain , somite , [+]

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  Figure 5. Neuronal expression for non-transcription factor candidate target genes. (A-C) pcdh8 is expressed in medial and intermediate stripes (arrowheads) of primary neurons, in one posterior stripe between the two stripes of primary neurons, and in the anterior domain of the neural plate at stage 15, and in the spinal cord (white arrows) and some domains in the brain at stages 23 and 28. (D-F) peripherin is expressed in the trigeminal placodes (yellow arrows) and three stripes of primary neurons (arrowheads) at stage 15, and in the trigeminal placodes, olfactory placodes (black arrow), spinal cord, retina and many domains in the brain at stages 23 and 28. (G-I) greb1 is expressed as a band in the prospective midbrain/hindbrain region at stage 15, and in the midbrain/hindbrain region and spinal cord at stage 23, and in the olfactory placodes, spinal cord, and many domains in the brain at stage 28. (J,K) nf-m is not expressed at stage 15, but at stages 23 and 28 it is expressed in the trigeminal placodes and spinal cord. (L-N) kcnk5 is weakly expressed from anterior to posterior along the dorsal midline, with stronger expression in the anterior end of the neural fold at stage 15. It is expressed in retina, otic placode, and several domains in the brain at stages 23 and 28, and in spinal cord at stage 28. (O-Q) activin beta b is expressed in two bands in the prospective midbrain/hindbrain region and diffusely throughout the anterior neural plate at stage 15, and it is expressed in the retina and some brain domains at stages 23 and 28. Stage 15 embryos show dorsal views, and stages 23 and 28 embryos show lateral views.

Gene Clone Species Stages Anatomy
greb1.L image:5569934 laevis NF stage 15 olfactory placode , neural plate , presumptive paraxial mesoderm

  greb1 (growth regulation by estrogen in breast cancer 1) gene expression in Xenopus laevis embryos, NF stage 15, as assayed by in situ hybridization. Dorsal view, anterior left.

Gene Clone Species Stages Anatomy
greb1.L image:5569934 laevis NF stage 23 somite , ventral blood island , involuted dorsal mesoderm , mesenchyme , neural tube , [+]

  greb1 (growth regulation by estrogen in breast cancer 1) gene expression in Xenopus laevis embryos, NF stage 23, as assayed by in situ hybridization. Lateral view: dorsal up, anterior left.