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gli3xenopus optic stalk [+] 

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Experiment details for gli3

Qiu R et al. (2009) Assay

The role of miR-124a in early development of the Xenopus eye.

Gene Clone Species Stages Anatomy
gli3.L laevis NF stage 20 optic stalk

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  Fig. 4. miR-124a and its candidate targets. (A) An evolutionarily conserved miR-124 target site is found at the 3′ UTR of Lhx2 mRNAs in different species. Seed sequences are marked in red. Homologous sites are marked by asterisks. (B) Expression of the predicted targets of miR-124a: Lhx2, Hairy2, Gli3, NeuroD1 and Otx2 were detected by whole mount in situ hybridization. Embryos were injected with 0.05 pmol miR-124a precursor molecules into one dorsal-animal blastomere at the 8-cell stage and collected at stage 13 (anterior view), stage 20 (anterior view) or stage 33 (lateral view and dorsal view). The pink arrowhead indicates the reduced expression of Lhx2 on the injected side. Scale bars: 500 μm. (C) Luciferase assays were carried out in HEK293 cells using the pCS2-Luc-3′ UTR reporters of Lhx2, Gli3, and the positive (anti-miR-124a) and negative (Pax6) controls. Luciferase activity after transfection of the miR-124a precursor (pre-124a) or control precursor (pre-control) was normalized by that of the control cells without precursor transfection. Data are expressed as the means of three independent transfections ±SD, each carried out in triplicate. Asterisks indicate where the miR-124a transfected group was significantly different from the control precursor group (P < 0.05). (D) In situ hybridization detection of miR-124a and Lhx2 expression in Xenopus embryos at stage13 (anterior view), stage 20 (anterior view) and stage 33 (transection at the eye-level). Scale bars: 300 μm (stage 13, stage 20); 100 μm (stage 33).