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Fig. 6. Vegetal expression of Furin and SPC6 can restore a normal dorsal axis to UV-ventralized Xenopus embryos. UV-treated embryos were injected in a vegetal blastomere at the 4 cell (B, D and E) or 8-cell stage (C). (A) Control noninjected, non-UV-irradiated stage 37/38 embryos. (B) UV-irradiated embryos injected with 1 ng GFP mRNA showing DAIs of 0–2. (C) UV-irradiated embryos injected vegetally with 450 pg Furin and 450 pg SPC6 mRNA at the 8-cell stage showing DAIs ranging from 2 to 3. (D) UV-irradiated embryos injected vegetally with 450 pg Furin and 450 pg SPC6 mRNA at the 4-cell stage exhibiting DAIs of 4–5. All embryos were allowed to develop until control non-UV-treated embryos reached stage 37/38. The representative rescued embryos shown in panel D have anterior structures, including eyes, cement gland, and neural crest-derived pigmented cells. (E) The frequency distribution of DAI scores for a representative experiment is shown, each dot corresponds to a single embryo. These experiments were performed six times, in which 0/150 of the control, UV-irradiated embryos had a DAI > 2. (F). Whole mount hybridization in situ of Vg1 mRNA in hemisected control and UV-irradiated stage 9 Xenopus embryos. |
