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Fig. 1. GATA4 and GATA6 are expressed in migratory leading edge mesendoderm throughout gastrulation. (A–L) In situ hybridisation on embryos just prior to (stage 9), during (stages 10.25 and 12) and at the end of (stage 13) gastrulation. Serial sections were hybridised with probes for GATA6, GATA4 and the mesodermal marker Xbra. (M–O) In situ hybridisation showing coexpression of GATA6 with cerberus and Xhex (markers of leading edge mesendoderm) during gastrulation. The vegetal pole is to the bottom and dorsal is to the right in all panels except J–L, where dorsal is towards the top and anterior is left. Arrows in (A) and (B) denote suprablastoporal endoderm, where punctate staining is visible in a few cells. Arrow in (J) denotes archenteron roof. All sections are sagittal and 10 μm. |