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Figure 2. Fli1 Is Required Upstream of Gata2 for the Definitive Hemangioblast Program(A–L) Fli1 requirement for the definitive hemangioblast. Xenopus embryos injected with Fli1 MO in the VMZ at the 4-cell stage were processed for WISH at stage 26. Note remaining DLP signal in (B) marks the pronephric duct (PND). Red outlines hemangioblasts, black outlines PND. Black arrows, DLP; red arrows, pVBI.(M–T) Gata2 morphants confirm that Fli1 is at the top of the definitive hemangioblast hierarchy. Embryos injected with Gata2 MOs in the VMZ at the 4-cell stage were processed for WISH for expression in the DLP (arrows) at stages shown. |
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Figure 1. Fli1 Is Required Upstream of Gata2 for the Primitive Hemangioblast Program(A–L) Fli1 requirement for the primitive hemangioblast. Xenopus embryos injected with Fli1 MO in the DMZ at the 4-cell stage were processed for in situ hybridization at stage 17, either as whole mounts (WISH) (A–H, J, K) or as sections (I, L).(M–X) Gata2 morphants and rescue experiments confirm that Fli1 is at the top of the primitive hemangioblast hierarchy. Gata2 MOs were injected into the DMZ at the 4-cell stage and embryos were processd for WISH at stages 16/17. Expression of SpiB (S–U) and Runx1 (V–X) was partially rescued by coinjection of mRNA for Scl, Lmo2, and Gata2.Arrows mark the primitive hemangioblast population. Proportion of embryos with phenotype shown indicated in top right corner. |