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FIG. 2. Whole embryo in situ analysis using an ant isense digoxigenin-labeled GATA·2 RNA probe. (A) Embryos include stages 9, 13, 20, 24, 34,
and 39. Note the ventral expression of GATA-2 RNA at stage 13 and localization of GATA-2 to the ventral blood island region by stage 24. In
stage 34 and 40 embryos, GATA-2 is also expressed in the central nervous system. (B-E) Tissue sections of whole embryo in situ analysis with a
GATA-2 RNA probe. (B) sagittal section showing GATA-2 expression in animal pole (arrows) of stage 11 embryo. (C) Ventral expression
(arrows) at stage 13; sagittal section. (D) Expression is more prominen t in inner layer by stage 20 (ventral region of transverse section). (E)
GATA·2 expression in mesodermal layer of transverse midsection at stage 26 (arrow points to most ventral region). |
