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Figure 1. Ventral (A) and Dorsal (B) Marginal Zone Contributions to Primitive and Definitive Blood Compartments (A) Time course from stage 17 to stage 35 showing ﰇ-gal localization in VMZ injected embryos. Numbers at the top right corner in- dicate stage of development. 17a, dorsal view. 17b, 22b, and 26a, ventral views. 22a, 28, and 35, lateral views. Anterior is to the left in all cases. Black arrows indicate the position of the DLP.
(B) Time course from stage 17 to stage 35 showing ﰇ-gal localization in DMZ injected embryos. Numbers at the top right corner in- dicate stage of development. 26a shows a benzyl benzoate/benzyl alcohol cleared em- bryo. Notice total absence of ﰇ-gal staining in the DLP region (black arrow). 26c, transverse section at the level of the anterior VBI of em- bryo in 26b. 26d, transverse section of a stage 26 embryo showing GATA-2 expression in the region of the anterior VBI. 17a, dorsal view. 17b, 22b, and 26b, top embryo, ventral views. 22a, 26a, 26b bottom embryo, and 35, lateral views. Ventral is to the bottom in all cases. In whole mounts anterior is to the left. Black arrow indicates the position of the DLP. Red arrows indicate B-gal localization in the ante- rior VBI. |
