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foxo6xenopus spinal cord 

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Experiment details for foxo6



FoxO genes are dispensable during gastrulation but required for late embryogenesis in Xenopus laevis.

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  Fig. 2. Expression of FoxO genes. (A) Temporal expression patterns of FoxO1, FoxO3, FoxO4 and FoxO6 genes during Xenopus embryogenesis and relative expression in adult tissues. Amount of transcripts was analyzed by semi-quantitative RT-PCR using RNA isolated from embryos at indicated developmental stages (Nieuwkoop and Faber, 1967) or from adult tissues. Expression levels are shown in relative units. Histone H4 was used as internal control. (B-M) Spatial patterns analyzed by whole mount in situ hybridization of FoxO4 (B-H) and FoxO6 (I-M). (B) 2-cell stage, lateral view. (C) Stage 17, anterior view. (D) Stage 21, anterior view. (E) Stage 23 embryo hybridized with a FoxO4 sense probe, anterior view. (F) Magnification of the head of an embryo, stage 29. (G) Stage 35/36, lateral view. (H) Transversal section of the head, stage 38. (I) Stage 22, anterior view. (J) Stage 27, lateral view. (K) Magnification of the head at stage 28, anterior view. (L) Stage 32, lateral view. (M) Stage 30, anterior view. Red arrows indicate the eye field, yellow arrows indicate the olfactory pits. ba, branchial arches; hg, hatching gland; hm, head mesenchyme; l, lens; nt, neural tube; ov, otic vesicle; pd, pronephric duct; pl, presumptive lens; pr, presumptive retina; r, retina.