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Fig. 7. BMP depletion rescues patterning defects in tsg morphants. Expression of region-specific markers were analyzed at stage 25 in uninjected embryos (A, E, I, M, Q, U, Y), and in embryos injected with 10 ng of tsg MO (B, F, J, N, R, V, Z), 20 ng each of BMP4 and BMP7 MOs (C, G, K, O, S, W, AA) or a combination of 10 ng tsg MO and 20 ng of BMP4 and BMP7 MOs (D, H, L, P, T, X, BB). Markers assayed included the midbrain hindbrain boundary marker en-2 (E H), the hindbrain marker krox-20 (I L), the spinal cord marker hoxb 9 (M P), the forebrain markers Xbf-1 (Q T) and eomesodermin (U X) and the ventral marker sizzled (YB). All views are lateral with anterior to the left and dorsal to the top. |
