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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hesx1
|
|
laevis
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NF stage 14
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neuroectoderm outer layer
,
pre-chordal neural plate
,
neural plate
,
anterior neural fold
,
anterior placodal area
|
pax6
|
|
laevis
|
NF stage 18
|
diencephalon
,
eye primordium
,
neural fold
|
sox3
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
neural plate
|
otx2
|
|
laevis
|
NF stage 11
|
dorsal marginal zone
,
involuted dorsal mesoderm
|
otx2
|
|
laevis
|
NF stage 15
|
neuroectoderm
,
pre-chordal neural plate
,
neural plate
,
optic field
|
en2
|
|
laevis
|
NF stage 16
|
presumptive midbrain-hindbrain boundary
|
egr2
|
|
laevis
|
NF stage 16
|
rhombomere
,
rhombomere R3
,
rhombomere R5
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acta4
|
|
laevis
|
NF stage 16
|
paraxial mesoderm
|
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Fig. 4. δXTcf3-mediated expansion of the neural plate can be uncoupled from effects on A patterning. (A) Embryos injected into one cell at the eight-cell stage with mRNA encoding δXTcf3 show expanded expression of Sox3 along the entire A axis (i, ii). However, sibling embryos show little or no expansion of Otx expression (iii, iv); note the lineage tracer, β-gal (light blue stain) along the entire A axis and highlighted by red arrow. (B) At doses of δXTcf3 RNA that expand Sox3 (i), the mesoderm marker Muscle Actin (ii) appears relatively unaffected on the injected side (arrow). A neural markers show normal expression on the injected side (arrow) relative to contralateral control. Markers examined are (iii) Krox20; (iv) En-2; (v) Pax6; (vi) BF-1: (vii) Xanf (viii) Otx with "p." denoting probe used.
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