|
Gene |
Clone |
Species |
Stages |
Anatomy |
foxf1.L
|
|
laevis
|
NF stage 19
|
neural plate
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
,
anterior neural ridge
|
foxf1.L
|
|
laevis
|
NF stage 23
|
forebrain
,
mandibular crest
,
hyoid crest
,
cranial neural crest
,
anterior dorsal lateral plate region
|
foxf1.L
|
|
laevis
|
NF stage 26
|
mandibular crest
,
hyoid crest
,
cranial neural crest
,
head
,
anterior dorsal lateral plate region
|
foxf1.L
|
|
laevis
|
NF stage 27
|
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
eye
,
olfactory region
|
foxf1.L
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
[+]
|
foxf1.L
|
|
laevis
|
NF stage 31
|
otic vesicle
,
pronephric duct
,
pharyngeal arch
,
eye
,
lateral plate mesoderm
,
[+]
|
foxf1.L
|
|
laevis
|
NF stage 35 and 36
to
NF stage 39
|
brain
,
head region
,
neural crest
,
mesoderm
,
ventral blood island
,
[+]
|
foxf1.L
|
|
laevis
|
NF stage 41
|
pharyngeal arch
,
cardiovascular system
,
lateral plate mesoderm
,
blood vessel
,
anterior dorsal lateral plate region
,
[+]
|
|
|
Fig. 2. Temporal and spatial expression of XFD-13/13' during Xenopus development for the temporal pattern, total RNA was subjected to RT-PCR (upper panel). The first lane (Oo) corresponds to RNA from mature oocytes, the last lane to a control assay lacking RNA. Histone H4 is used as an internal control. Stage classification is done according to Nieuwkoop and Faber (1975). The spatial pattern was analysed by whole mount in situ hybridizations. Embryos at stages 19 (A), 23 (B), 26 (C), 27 (D), 29 (E), 31(F), 36 (G), 39 (H) and 41 (I) and transversal sections of embryos at stages 24 (J), 32 (K) and 39 (L and M(higher magnification)) are shown. A horizontal section through a stage 32/33 embryo is shown in (N). dlp, dorsolateral plate; lb, lateral border of neural plate; n,notochord; nc, neural crest cells; v, vessel-like structures. |