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Fig. 2. Expression of FoxE1 in Xenopus embryos. (A-F) Lateral (A,C-F) and anterior (B) views of whole mount in situ hybridized with antisense probe for FoxE1. (A,B) Stage 18, (C) Stage 24, (D) Stage 27, (E) Stage 33/34 and (F) Stage 35/36. (GL) Comparison of FoxE1 expression with markers of the pituitary (H,I) and thyroid development (K,L). (G) Anterior view of a st 35/36 embryo hybridized with FoxE1. (H,I) Anterior views of tailbud stage embryos hybridized with antisense riboprobes for Xanf2 and POMC, respectively. (J,K,L) Lateral view of tailbud stage embryos hybridized with antisense riboprobes for FoxE1, Nkx2.1 and FoxE4 (Xlens1), respectively. Arrowheads denote the position of thyroid expression. (M-O) Section in situ hybridization of st 38 embryos hybridized with antisense probes for FoxE1 (M, O) or Nkx2.1 (N). (M) Expression of FoxE1 in the thyroid (arrowhead) and pharyngeal endoderm. No expression is observed in the lens (open arrowhead). (N) Expression of FoxE1 in branchial arch mesoderm (arrow) and pharyngeal endoderm. (O) Expression of Nkx2.1 in the thyroid (arrowhead). B, brain; E, eye; H, heart; N, notochord; OV, otic vesicle; P, pharynx. |