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Fig. 2. Xfd-12′ is expressed during medial neural plate morphogenesis. Whole-mount in situ hybridization was used to reveal the spatio-temporal expression pattern of Xfd-12′. (A) Stage 9; expression is initiated in the presumptive dorsal marginal zone. (B) Stage 10.5; expression increases in the dorsal circumblastoporal zone. (C,D) Stages 11 and 11.5; expression constricts in the dorsal midline. (E,J) Stage 12, sagittal and transverse sections; Xfd-12′ expression is restricted to the neuraxial midline. (F,G) Stages 12 and 12.5; strong expression in the prospective floorplate and the dorsal periblastoporal zone. (H,I) Stages 13 and 14; down-regulation in the rostral neuraxial midline. (K) Stage 17; expression remains high in the dorso-posterior region. (L) Stage 25; caudal expression in the tailbud. (M–O) Stage 25, frontal, sagittal and transverse sections; expression is observed in the chordoneural hinge (ch) and the tip of the posterior wall (pw). From stage 12 onwards expression is in addition found in the mid-hindbrain junction. Black arrowheads point to the lateral extent of dorsal circumblastoporal expression during neuraxial constriction and red arrowheads point to mid-hindbrain expression. Embryos are viewed (A,B) vegetally with dorsal up, (C,D) dorso-vegetally, (F–I) dorsally with anterior up, (K) dorsally with anterior left and (L) laterally with anterior left. |
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Fig. 6. Medial neural plate progenitors become localized to the trunk organizer. Comparative expression analysis at peri gastrulation stages. Serial sections of single stage 9 blastula (A,C,E,G) and stage 10.5 gastrula (B,D,F,H,I) embryos that were stained by in situ hybridization for cerberus (A,B), pintallavis (C,D), Xbra (E,F), Xfd-12′ (G,H) and goosecoid (I) expression. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
sox2
|
|
xenopus
|
NF stage 10
to
NF stage 12.5
|
ectoderm
,
nervous system
,
neural plate
,
dorsal
,
epithelial cell
|
foxa4
|
|
xenopus
|
NF stage 10
to
NF stage 12.5
|
mesoderm
,
notochord
,
dorsal marginal zone
,
blastopore lip
,
axial mesoderm
,
[+]
|
shh
|
|
xenopus
|
NF stage 10
to
NF stage 12.5
|
notochord
,
floor plate
|
|
|
Fig. 3. Xfd-12′ marks medial neuraxial progenitors prior to shh activation in the floorplate. Whole-mount in situ hybridization analysis was used to compare the spatio-temporal expression of pintallavis (A,E,I,M), shh (B,F,J,N), sox-2 (C,G,K,O) and Xfd-12′ (D,H,L,P) during gastrulation at stage 10.5–11 (A–D), stage 11.5–12 (E–H), stage 12.5–13 (I–L) and neurulation at stage 14 (M–P). Arrows point to the dorsal blastopore. Embryos in (A–D) are viewed vegetally with dorsal up, whereas all other embryos are viewed from the dorsal perspective with anterior up. |