| |
Figure 12. FoxA4MO reproduced the effects on anterior neural markers in embryos in which mesoderm induction was blocked with Cer-S.(A–D) Expression of foxA4 in sibling controls (A, C) or in embryos injected with Cer-S mRNA (B, D), analysed at stage 9 (A, B) or at stage 11 (C, D). After blocking mesoderm induction, foxa4 expression persisted in the BCNE (B) (100%, n = 15) but was suppressed from the axial mesoderm (D) (100%, n = 7). (E, F) MyoD expression at neural plate stage in a sibling control (E) and in an embryo injected with Cer-S. Mesoderm was suppressed in 100% of the injected embryos analyzed with myoD (100%, n = 8). (G–I) Expression of Xanf1 and en2 at neural plate stage in a sibling control (G), and in embryos in which mesoderm induction was blocked with Cer-S and were injected at the 1-cell stage with either 20 ng of CtrlMO (H) or 20 ng of FoxA4MO (I). Embryos injected with CtrlMO + Cer-S showed expression of Xanf1 (yellow arrow) and en2 (black arrow) (H, 100%, n = 8). In embryos injected with FoxA4MO + Cer-S, Xanf1 was expanded and up-regulated (yellow arrow) and en2 was down-regulated (black arrow) (I, 100%, n = 10) in comparison to embryos injected with CtrlMO + Cer-S (H). (J–L) Expression of otx2 at neural plate stage in a sibling control (J), and in embryos in which mesoderm induction was blocked with Cer-S and were injected at the 1-cell stage with either 20 ng of Ctrl MO (K) or 20 ng of FoxA4MO (L). Embryos injected with CtrlMO + Cer-S showed expression of otx2 (K, 100%, n = 4). In embryos injected with FoxA4MO + Cer-S, otx2 was down-regulated (L, 100%, n = 20) in comparison to embryos injected with CtrlMO + Cer-S (K). |
