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Fig. 6. Xhox3 and XFD-I transcription in embryos injected with BMP-2 mRNA. Xenopus embryos at the four-cell stage were injected with each 1 ng of BMP-2 mRNA or BMP-2delta Pst I mRNA into the two dorsal blastomeres. (A) Total RNA (equivalent of 5 embryos) of BMP-2 injected (BMP-2) or uninjected embryos (control) at stage 12 was analysed by RNase protection with antisense RNA transcribed from a 750 bp Eco RI fragment of Xhox3 (Ruiz i Altaba and Melton, 1989a, 1989b). a 484 bp Eco RI/Bgl II fragment of XFD-1 (Kn6chel et al., 1992) or a 311 bp Pvu II/Pst I fragment of EF-lα (pXEF7, P�ting et al., 1990). Yeast tRNA was used as control. (B) Embryos were fixed at stage 11. Whole mount in situ hybridization was performed with antisense RNA transcribed from a 484 bp Eco RI/Bgl II fragment of XFD-1. |
