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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gli1
|
|
laevis
|
NF stage 15
to
NF stage 17
|
neuroectoderm
,
neural plate
,
neural tube
,
pre-chordal neural plate border
,
chordal neural plate border
,
[+]
|
pax3
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
neural plate
,
neural plate border
,
anterior neural ridge
|
sox2
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
neural plate
|
gli3
|
|
laevis
|
NF stage 16
to
NF stage 17
|
neuroectoderm
,
neural plate
,
anterior neural fold
,
neural tube
,
pre-chordal neural plate border
,
[+]
|
foxa4
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
floor plate
,
neural tube
|
shh
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
neural groove
,
neural plate
,
floor plate
,
neural tube
|
shroom3
|
|
laevis
|
NF stage 14
to
NF stage 15
|
neuroectoderm
,
neural plate
,
anterior neural fold
,
neural tube
,
neural plate border
|
tubb2b
|
|
laevis
|
NF stage 14
to
NF stage 15
|
neuroectoderm
,
neural plate
,
Rohon-Beard neuron
,
neuron
|
ncam1
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
neural plate
|
cdh2
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
neural plate
|
ptch2
|
|
laevis
|
NF stage 15
to
NF stage 16
|
neuroectoderm
,
neural groove
,
neural plate
,
neural tube
|
krt12.4
|
|
laevis
|
NF stage 14
|
epidermis
,
non-neural ectoderm
|
|
|
Fig. S3. In situ hybridization analysis of xMID morphants. (A-Z) Dorsal views of unilaterally injected morphants at stage 14-15; anterior is to the top. Morpholinos were injected into the right dorsal blastomere at the four-cell stage. Sox2 (A,B), NCAM (C,D) and N-cadherin (E,F) are pan-neural markers. Epidermal keratin (G,H) is an epidermal marker. HNF3β (FoxA2a) (I,J) and Pintallavis (FoxA4a) (K,L) are floor-plate markers. N-tubulin (M,N) is a marker for differentiated neurons. Shroom3 (O,P) is the essential regulator of apical constriction. Pax3 (Q,R) is a dorsal neural marker. Sonic hedgehog (Shh) (S,T), a key molecule for neural dorsoventral patterning, is expressed in the floor plate. Ptc2 (U,V) and Gli1 (W,X) are downstream targets in the Shh pathway. Gli3 (Y,Z) is a transcription factor mediating the Shh pathway. The expression patterns of all the markers in the xMID morphants were similar to those in control-Mo-injected embryos, except that the expression domains were wider than in the controls at given stages because of the delayed neural tube closure. |
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foxA2 (forkhead box A2) gene expression in Xenopus laevis embryo, via in situ hybridization, NF stage 17, dorsal view, anterior up. |