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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
fmr1	xenopus

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Experiment details for fmr1

Gessert S et al. (2010) Assay

FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development.

Gene	Clone	Species	Stages	Anatomy
fmr1.L		laevis	NF stage 15	neuroectoderm , pre-chordal neural plate , neural plate , anterior
fmr1.L		laevis	NF stage 20	mandibular crest , hyoid crest , branchial crest , eye primordium , cranial neural crest , [+] neural tube , migratory neural crest cell
fmr1.L		laevis	NF stage 25	mandibular crest , hyoid crest , eye , otic placode , eye primordium , [+] posterior neural tube , anterior neural tube , cranial neural crest , neural tube , migratory neural crest cell , anterior branchial crest , posterior branchial crest
fmr1.L		laevis	NF stage 28	otic vesicle , brain , spinal cord , mandibular crest , hyoid crest , [+] eye , olfactory region , migratory neural crest cell , anterior branchial crest , posterior branchial crest
fmr1.L		laevis	NF stage 33 and 34	lens , retina , brain , spinal cord , mandibular crest , [+] hyoid crest , pronephric nephrostome , pronephric duct , ciliary marginal zone , pharyngeal arch , mandibular arch , hyoid arch , branchial arch , eye , pronephric kidney , stem cell , tail tip

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Suppl. Fig. 1. Spatial expression pattern of FMR1 and FXR1. A. WMISH detecting FMR1. At stage 15 a weak FMR1 expression can be detected in anterior neural tissue (white arrowhead). At stage 20, FMR1 transcripts can be visualized in the neural tube (red arrow), the migrating cranial NC cells (white arrow) and the eye anlage (black arrow). Expression in the eye and the NC is maintained at later stages 25, 28, and 33. FMR1 is also expressed in the otic vesicle (stage 25, green arrow) and the pronephric nephrostomes (stage 33, green arrowheads). At stage 33 FMR1 is expressed in the lens and the ciliary marginal zone of the eyes (left vibratome section on the level of the white dotted line) and parts of the pharyngeal arches (right vibratome section on the level of the yellow dotted line, white arrow). B. Spatial expression pattern of FXR1. In all examined stages, FXR1 transcripts can be detected in the somites (e.g. stage 28 red arrowhead) and at stage 40 in the migrating hypaxial muscle cells (red arrowhead). At stage 20, FXR1 is additionally expressed in the anterior neural tissue (white arrowhead).

Gene	Clone	Species	Stages	Anatomy
fmr1.L		laevis	NF stage 15	neuroectoderm , neural plate , anterior

fmr1 (fragile X mental retardation 1) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 15, anterior view, dorsal up.

Gene	Clone	Species	Stages	Anatomy
fmr1.L		laevis	NF stage 20	mandibular crest , hyoid crest , branchial crest , eye primordium , anterior neural tube , [+] cranial neural crest , neural tube , migratory neural crest cell

fmr1 (fragile X mental retardation 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 20, anterior view, dorsal up.

Gene	Clone	Species	Stages	Anatomy
fmr1.L		laevis	NF stage 25	brain , mandibular crest , hyoid crest , neural crest , eye , [+] otic placode , eye primordium , posterior neural tube , anterior neural tube , cranial neural crest , neural tube , migratory neural crest cell , anterior branchial crest , posterior branchial crest

fmr1 (fragile X mental retardation 1 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, lateral view, anterior right, dorsal up.

Gene	Clone	Species	Stages	Anatomy
fmr1.L		laevis	NF stage 28	otic vesicle , brain , spinal cord , mandibular crest , hyoid crest , [+] eye , cranial neural crest , olfactory region , anterior branchial crest , posterior branchial crest

fmr1 (fragile X mental retardation 1) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 28, lateral view, anterior right, dorsal up.

Gene	Clone	Species	Stages	Anatomy
fmr1.L		laevis	NF stage 33 and 34	lens , retina , brain , spinal cord , pronephric nephrostome , [+] pronephric duct , ciliary marginal zone , pharyngeal arch , mandibular arch , hyoid arch , branchial arch , eye , pronephric kidney , stem cell , olfactory region , tail tip

fmr1 (fragile X mental retardation 1) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 33 & 34, lateral view, anterior right, dorsal up.( far left); section through eye (plane of white dashed line) center; horizontal section through pharyngeal region (plane of yellow dashed line).