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flrt3xenopus   

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Experiment details for flrt3

Unc5B interacts with FLRT3 and Rnd1 to modulate cell adhesion in Xenopus embryos.

Unc5B interacts with FLRT3 and Rnd1 to modulate cell adhesion in Xenopus embryos.

Gene Clone Species Stages Anatomy
flrt3.L laevis NF stage 9 marginal zone , blastocoel roof , vegetal yolk mass , blastocoel , sensorial layer , [+]
flrt3.L laevis NF stage 10.5 marginal zone , involuting marginal zone , involuted dorsal mesoderm , involuted ventral mesoderm , upper blastopore lip

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  Figure 3. Unc5B-FLRT3 interaction in Xenopus tropicalis morphogenesis.(A) Developmental quantitative RT-PCR analysis of FLRT3 and Unc5A-D expression. (B–C) Expression patterns of FLRT3 and Unc5B at blastula stage 9 and gastrula stage 10.5. The dorsal blastopore lip is indicated with an arrow. DM, dorsal mesoderm. (D) Representative phenotypes of embryos injected with FLRT3 and/or Unc5B Morpholinos (MOs). A mixture of two MOs per gene targeting the ATG and the UTR regions was injected (10 ng each). A stunted axis was observed in 0% (n = 29), 3% (n = 34), 7% (n = 28) and 100% (n = 31) of the injected embryos, respectively. Lower row: whole mount in situ hybridization of Xnot at late gastrula stage 12.