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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
unc5b
|
|
laevis
|
NF stage 9
|
animal cap
,
animal cap inner layer
,
animal hemisphere
,
blastocoel roof
,
vegetal yolk mass
,
[+]
|
unc5b
|
|
laevis
|
NF stage 10.5
|
animal cap
,
animal cap inner layer
,
involuting marginal zone
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
,
[+]
|
|
|
Figure 3. Unc5B-FLRT3 interaction in Xenopus tropicalis morphogenesis.(A) Developmental quantitative RT-PCR analysis of FLRT3 and Unc5A-D expression. (B–C) Expression patterns of FLRT3 and Unc5B at blastula stage 9 and gastrula stage 10.5. The dorsal blastopore lip is indicated with an arrow. DM, dorsal mesoderm. (D) Representative phenotypes of embryos injected with FLRT3 and/or Unc5B Morpholinos (MOs). A mixture of two MOs per gene targeting the ATG and the UTR regions was injected (10 ng each). A stunted axis was observed in 0% (n = 29), 3% (n = 34), 7% (n = 28) and 100% (n = 31) of the injected embryos, respectively. Lower row: whole mount in situ hybridization of Xnot at late gastrula stage 12. |