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Fig. 2. In situ hybridisation in Xenopus wild type and N1 line after induction of extra limbs. (A-C)lmx1 expression in the wild-type limb is restricted to the dorsal mesenchyme (A) but is present in the entire mesenchyme in the endogenous limb of the N1 line (B,C), indicating a dorsalisation of the limb; the extra limb shows normal lmx1 expression restricted to the dorsal mesenchyme (C). (D-F) en1 expression is restricted to the ventral ectoderm in the wild type (D) but is absent in the N1 endogenous limbs (E), whereas extra limb shows normal expression of en1 (F). (G-I)Expression of fgf8 is restricted to the AER in wild-type limbs (G) but is expressed in a broader domain in N1 endogenous limbs while normally expressed in the AER of the extra limbs (H,I). Arrowheads indicate endogenous limbs; arrows indicate extra limbs. |
