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Figure 7. Expression of fgf-8 expanded in the limb bud with a 180-degree rotation. The stage 52 limb bud tip (amputated at the presumptive tarsus level) was transplanted to the stump amputated at the presumptive femur level with (B) or without (D) 180-degree rotation. Five days after the operation, the samples were processed for hybridization with fgf-8 cDNA. Manipulated (B,D) and control (A,C) limbs are left and right, respectively. The black arrows indicate the normal and expanded expression of fgf-8 in apical ectodermal ridge and the black arrowheads in (B) indicate the ectopic expression of fgf-8 in the stump. The red arrowheads indicate the amputation plane. a, anterior; p, posterior. Scale bar = 200 μm in D (applies to A–D). |
