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Fig. 7. Developmental expression of putative hindbrain-derived otic-inducing factors. (A) Fgf3, Fgf8, Wnt1 and Wnt8 are expressed in a region of NP corresponding to NP2 domain at the early neurula stage; dorsal views, anterior to top. (B) Pax8 otic expression at stage 15 (black arrow). (C) Double in situ hybridization showing the relationship of expression of Pax8 (black arrows; green staining) and Fgf3, Fgf8, Wnt1 and Wnt8 (white arrows; purple staining) at the neurula stage. Lateral views, anterior to the left. At this stage Wnt8 is also expressed outside of the hindbrain in a region of the ectoderm presumably corresponding to the neural crest. (D) Real-Time RT-PCR analysis of Fgf3, Fgf8, Wnt1 and Wnt8 expression in various segments of NP (NP1, NP2 and NP3) shortly after dissection. All four ligands are enriched in NP2 domain. WNP; whole NP. |
