|
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf2
|
fgf2-har396
|
tropicalis
|
NF stage 10.5
|
mesoderm
,
marginal zone
|
fgf2
|
fgf2-har396
|
tropicalis
|
NF stage 15
|
paraxial mesoderm
|
fgf2
|
fgf2-har396
|
tropicalis
|
NF stage 23
|
head region
,
presomitic mesoderm
|
fgf2
|
fgf2-har396
|
tropicalis
|
NF stage 28
|
head region
,
presomitic mesoderm
,
eye
|
fgf2
|
fgf2-har396
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
neural crest
,
mesoderm
,
branchial arch
,
tail bud
,
[+]
|
fgf2
|
fgf2-har396
|
tropicalis
|
NF stage 40
|
otic vesicle
,
head mesoderm
,
branchial arch
,
tail bud
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf8
|
|
tropicalis
|
NF stage 10.5
|
marginal zone
|
fgf8
|
|
tropicalis
|
NF stage 15
|
neuroectoderm
,
pre-chordal neural plate
,
neural crest
,
neural plate
,
cranial neural crest
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 23
|
forebrain
,
midbrain-hindbrain boundary
,
neuroectoderm
,
neural crest
,
presomitic mesoderm
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 28
to
NF stage 35 and 36
|
forebrain
,
midbrain-hindbrain boundary
,
head region
,
mesoderm
,
somite
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 40
|
otic vesicle
,
forebrain
,
midbrain-hindbrain boundary
,
somite
,
pharyngeal arch
,
[+]
mandibular arch
,
hyoid arch
,
branchial arch
,
tail bud
,
chordoneural hinge
,
posterior wall of neurenteric canal
,
eye
,
paraxial mesoderm
,
heart primordium
,
pronephric kidney
,
neural tube
|
fgf4
|
|
tropicalis
|
NF stage 10.5
|
mesoderm
,
marginal zone
|
fgf4
|
|
tropicalis
|
NF stage 15
|
mesoderm
,
posterior
|
fgf4
|
|
tropicalis
|
NF stage 23
|
somite
,
tail bud
,
eye primordium
,
paraxial mesoderm
|
fgf4
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
midbrain-hindbrain boundary
,
somite
,
branchial arch
,
tail bud
,
[+]
|
fgf4
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
midbrain-hindbrain boundary
,
somite
,
branchial arch
,
tail bud
,
[+]
|
fgf4
|
|
tropicalis
|
NF stage 40
|
otic vesicle
,
midbrain-hindbrain boundary
,
head region
,
somite
,
branchial arch
,
[+]
|
fgf1
|
|
tropicalis
|
NF stage 10.5
|
marginal zone
|
fgf1
|
|
tropicalis
|
NF stage 15
|
embryo
,
dorsal
|
fgf1
|
|
tropicalis
|
NF stage 23
|
notochord
,
eye
|
fgf1
|
|
tropicalis
|
NF stage 28
|
olfactory placode
,
otic vesicle
,
forebrain
,
ventricular zone
,
eye
,
[+]
|
fgf1
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
hindbrain
,
ventricular zone
,
head region
,
[+]
|
fgf1
|
|
tropicalis
|
NF stage 40
|
fin
,
otic vesicle
,
forebrain
,
hindbrain
,
head
,
[+]
|
fgf6
|
|
tropicalis
|
NF stage 23
to
NF stage 35 and 36
|
head region
,
somite
,
paraxial mesoderm
|
fgf6
|
|
tropicalis
|
NF stage 40
|
head region
,
somite
,
paraxial mesoderm
|
|
|
Figure 2. Analysis of the spatial expression patterns of the fgf1, fgf4, and fgf8 subfamilies. A: Whole mount in situ hybridisations of fgf1 and fgf2 at the indicated stages. B: Whole mount in situ hybridisations of fgf4 and fgf6 at the indicated stages. C: Whole mount in situ hybridisations of fgf8. For stage 10.5, the images are vegetal views; for stage 15, the images are dorsal views; and for stage 23, 28, 35, and 40, the images are lateral views (anterior is left, posterior is right, dorsal is up, and ventral is down). A schematic representation of a Xenopus embryo at stage 28 with the different embryonic tissues labelled is presented in Supp. Figure S3. |
|
|
|
fgf2 (fibroblast growth factor 2 (basic)) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 15, dorsal view, anterior up. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf8
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
forebrain
,
midbrain-hindbrain boundary
,
presomitic mesoderm
,
somite
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
hindbrain
,
midbrain-hindbrain boundary
,
somite
,
[+]
|
fgf1
|
|
tropicalis
|
NF stage 23
|
notochord
|
fgf1
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
forebrain
,
neural tube
|
fgf1
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
anterior neural tube
,
neural tube
|
fgf1
|
|
tropicalis
|
NF stage 40
|
otic vesicle
,
forebrain
,
branchial arch
,
anterior neural tube
,
dorsal tail fin
,
[+]
|
fgf2
|
|
tropicalis
|
NF stage 28
|
presomitic mesoderm
|
fgf2
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
head mesoderm
,
branchial arch
,
tail bud
,
pronephric kidney
|
|
|
Figure 3. Cross section analysis of the spatial expression patterns of the fgf1, fgf4, and fgf8 subfamilies. Coronal sections of indicated fgfs from the whole mount in situ hybridisations shown in Figure 2. The black lines indicate the position of the sections shown; when multiple sections are shown, numbers correspond to the appropriate black line and, therefore, the section's position on the embryo. In all sections, dorsal is up and ventral is down. |
|
|
|
fgf2 (fibroblast growth factor 2 (basic))gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 23, lateral view, anterior left, dorsal up. |
|
Display additional annotations [+]
|
|
Figure 10. Detail analysis of the expression of the different fgf and fgfr genes in the otic vesicle. A Coronal sections (dorsal up) of whole mount in situ hybridisations staining within the otic vesicle for fgf1, fgf2, fgf3, fgf4, fgf8, fgf10 and fgf20 at stages 28, 35 and 40. B Coronal sections (dorsal up) of whole mount in situ hybridisations staining within the otic vesicle for fgfr1, fgfr2, fgfr3 and fgfr4 at stages 28, 35 and 40.
|
|
|
|
fgf2 (fibroblast growth factor 2 (basic)) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf2
|
|
tropicalis
|
NF stage 23
|
presomitic mesoderm
|
fgf8
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
forebrain
,
midbrain-hindbrain boundary
,
presomitic mesoderm
,
somite
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
hindbrain
,
midbrain-hindbrain boundary
,
somite
,
[+]
|
fgf1
|
|
tropicalis
|
NF stage 23
|
notochord
|
fgf1
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
forebrain
,
neural tube
|
fgf1
|
|
tropicalis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
anterior neural tube
,
neural tube
|
fgf1
|
|
tropicalis
|
NF stage 40
|
otic vesicle
,
forebrain
,
branchial arch
,
anterior neural tube
,
dorsal tail fin
,
[+]
|
|
|
Figure 3. Cross section analysis of the spatial expression patterns of the fgf1, fgf4, and fgf8 subfamilies. Coronal sections of indicated fgfs from the whole mount in situ hybridisations shown in Figure 2. The black lines indicate the position of the sections shown; when multiple sections are shown, numbers correspond to the appropriate black line and, therefore, the section's position on the embryo. In all sections, dorsal is up and ventral is down. |
|
|
|
fgf2 (fibroblast growth factor 2 (basic)) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 35 & 36, lateral view, anterior left, dorsal up.
|
|
|
|
fgf2 (fibroblast growth factor 2 (basic)) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 40, lateral view, anterior left, dorsal up.
|