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fat1xenopus   

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Experiment details for fat1

Giudetti G et al. (2014) Assay

Characterization of the Rx1-dependent transcriptome during early retinal development.

Gene Clone Species Stages Anatomy
fat1.L laevis NF stage 15 pre-chordal neural plate , neural plate , pre-chordal neural plate border , anterior neural ridge

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  Figure 4. Alteration of the expression of genes controlling migration/adhesion in Rx1 gain- and loss-of function experiments. A–I: Embryos injected unilaterally with control standard morpholino (CoMo; A, D, G), MoRx1 (B, E, H), or Rx1 (C, F, I) and analyzed at early neurula stage. Frontal views, dorsal to the top. The probes used are indicated in the top right corner of each panel. J: Relative quantification by RT-qPCR of Sh2d3c, Efnb1, and Fat1 in embryos injected with GFP alone (GFP), or coinjected with either control standard morpholino and GFP (CoMo) or MoRx1 and GFP (MoRx1). Error bars indicate SEM. *P < 0.05; **P < 0.01 (Student's t-test). No significant difference is observed between GFP and CoMo samples.Download figure to PowerPoint

Gene Clone Species Stages Anatomy
fat1.L laevis NF stage 16 optic field

  fat1 (FAT atypical cadherin 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 16, anterior view, dorsal up.

Gene Clone Species Stages Anatomy
fat1.L laevis NF stage 27 retina

  fat1 (FAT atypical cadherin 1) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up.