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Fig. 2. Expression pattern of XMeis3. (A) Whole-mount in situ hybridization with XMeis3 was performed on early neurula stage embryos (see Section 4). All embryos are oriented anterior to posterior, left to right. A single stripe of expression is seen in the neural plate (dorsal-lateral view). (B) XMeis3 expression in neurula stage embryos, dorsal view. Expression is observed in a patch of cells in the anterior hindbrain (corresponding to rhombomeres 2), followed by a gap; expression continues in the anterior spinal cord. (C,D) A dorsal-lateral (C) and lateral view (D) of late neurula - early tailbud stage embryos. The arrow (D) marks the ventral expression in the posterior hindbrain. (E) Double in situ hybridization with Krox-20 and XMeis3. XMeis3 is expressed anterior to the r3 Krox-20 stripe, but not posterior to the r5 stripe. Asterisks mark XMeis3 expression in r2 and r4. Arrows mark Krox-20 expression in r3 and r5. (F). Double in situ hybridization with En-2 and XMeis3. The arrow marks En-2 expression. XMeis3 is expressed posterior to the En-2 stripe at the midbrain-hindbrain junction. XMeis3 is not expressed in r1 as seen by the gap between the En-2 and XMeis3 expression domains. |
