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Fig. 3. Pangolin can replace XTcf-1 and XTcf-4, HyTcf can replace XTcf-4. (A) Expression of Xengrailed 2 (Xen2) in the isthmus organizer is reduced in XTcf-1 depleted embryos (T1Mo) and partially restored following co-expression of XTcf-4 (T1Mo+XTcf4), HydraTcf (T1Mo+HyTcf) and Pangolin (T1Mo+pan), but not after co-injection of Pop-1 (T1Mo+pop). 4 pMol XTcf-1 specific morpholino antisense oligonucleotide (T1Mo) was co-injected with 500 pg cDNA of the indicated Tcfs in the animal hemisphere of one blastomere in two-cell stage embryos. The asterisks mark the injected site. (B) Quantification of the in situ hybridization results shown in (A); N=number of analyzed embryos. (C) Expression of XTcf-4 in the midbrain is reduced in XTcf-1 depleted embryos and partially restored following co-expression of Pangolin, but not after co-injection of HydraTcf and pop-1. (D) Quantification of the in situ hybridization results shown in (C). (E) Expression of Xengrailed 2 (Xen2) in the isthmus organizer is reduced in XTcf-4 depleted embryos (T4Mo) and partially restored following co-expression of XTcf-4 (T4Mo+XTcf4), HydraTcf (T4Mo+HyTcf) and Pangolin (T4Mo+pan), but not after co-injection of Pop-1 (T4Mo+pop). 10 pMol XTcf-4 specific morpholino antisense oligonucleotide (T4Mo) was co-injected with 500 pg cDNA of the indicated Tcfs in the animal hemisphere of one blastomere in two-cell stage embryos. Quantification of the in situ hybridization results. |
