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Fig. 5. Embryonic spatial expression profile of S1P genes. Whole mount in situ hybridisation with DIG-labelled RNA probe was performed on embryos from stages 6-41 for S1P genes, except for S1P4. (A) Whole mount in situ hybridisation analysis of S1P1 expression. Dorsal and lateral views of a cleared embryo at stage 23 (i, ii), of uncleared embryos at stage 27 (iii), at stage 32 (v, vi), at stage 41 (vii, ix). Sagital section (ix of the head at stage 41. Tranverse sections (ix, ix at stage 41. The dotted lines through the embryo (ix) correspond to planes of the transverse sections. Dorsal view of a stage 27 (iv) and stage 41 (viii) embryo stained with En2 antisense probe. The arrow indicates the midbrain/hindbrain boundary marked by En2 expression. For the lateral views, dorsal is up and anterior is left. fb: forebrain; hb: hindbrain; mb; midbrain; ns: neural system; nt: neural tube; op: otic placode; sc: spinal cord. |
