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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
egr2
|
|
laevis
|
NF stage 19
|
neural tube
|
nuak1
|
|
laevis
|
NF stage 10
|
ectoderm
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
|
nuak1
|
|
laevis
|
NF stage 13
to
NF stage 21
|
ectoderm
,
epidermis
,
mesoderm
,
eye
,
sensorial layer of neurectoderm
,
[+]
|
nuak1
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
midbrain-hindbrain boundary
,
presomitic mesoderm
,
pharyngeal arch
,
mandibular arch
,
[+]
|
nuak1
|
|
laevis
|
NF stage 23
|
midbrain-hindbrain boundary
,
presomitic mesoderm
,
tail bud
,
eye
|
|
|
Fig. 1. xMAK expression is dynamically regulated during Xenopus development. (A) The analysis of xMAK expression at different developmental stages. Total RNA from embryos isolated at different developmental stages was used for RT-PCR. FGFR served as a loading control. RT-, no reverse transcriptase. (B-M) Spatial distribution of xMAK RNA revealed by whole-mount in situ hybridization of albino embryos at indicated stages. (B-E,J,K) xMAK antisense probe. (F,G,I,L,M) xMAK sense probe. (D) Anterior view; e, eye; MHB, midbrain-hindbrain boundary. (H) En2 and Krox20 probes, anterior view. En2 is expressed as a bright band at the MHB, located anterior to Krox20, which marks rhombomeres 3 and 5. (B,F) Animal pole view; (C,G) lateral view; (E,I) dorsal view, anterior is towards the left. (J-M) Lateral view, anterior is towards the left. (K) e, eye; ov, otic vesicle; ba, branchial arches; tb, tailbud. (N,O) A cross-section of a stage 17 neurula embryo after in situ hybridization with xMAK antisense probe. Staining is observed in the deep (sensorial) layer of epidermal ectoderm (magnified view shown in O) and in somitogenic mesoderm. |
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Display additional annotations [+]
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Fig. 7. MAK functions in regional brain patterning, but does not affect organizer markers. (A-O) MAK regulates midbrain-hindbrain boundary. Dorsal-animal region of four-cell embryos was injected with 50 ng of MOs or 2 ng of MAK RNAs, as indicated, together with nβgal RNA as a lineage tracer. At stage 20, Otx2, En2 and Gbx2 were assessed by whole-mount in situ hybridization. MAK MO inhibited Otx2 and En2 (D,E), but upregulated Gbx2 (F). MAK RNA expanded and posteriorly shifted Otx2 and En2 on the injected side (J,K), and inhibited Gbx2 (L). Red arrows indicate altered expression; black arrows indicate marker expression on the uninjected side. (P) MAK depletion or overexpression does not affect organizer marker expression. Two to four-cell embryos were injected twice in the dorsal margin with MAK MO or COMO (100 ng), β-catenin MO (30 ng) or indicated RNA (4 ng). RT-PCR was carried out with total RNA from stage 10 embryos. DNA fragments were separated in 6% SDS-polyacrylamide gel. EF-1α is a loading control. Uninj, uninjected embryos. RT-, no reverse transcriptase. |