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Fig. 8. miR-196a MO phenotype. A. The injection of miR-196a MO led to a strong eye phenotype on the injected side in a dose-dependent manner (black and white arrows). B. The early eye marker genes Rx1 and Pax6 were slightly affected upon loss of miR-196a function. C. The injection of 20 ng miR-196a MO resulted in a diffused and broadened expression pattern of Rx1, Pax6, Emx1, En2 and Krox20 at stage 23. D. Loss of miR-196a led to reduced cranial cartilage at stage 46 (black arrows). E. At stage 17, the expression of Slug and FoxD3 was strongly affected upon miR-196a depletion. E. FoxD3 at stage 20 and Krox20 and Twist at stage 23 were downregulated after injection of 20 ng miR-196a MO. For all shown experiments, quantitative representations are given. n = number of independent experiments; N = number of analyzed embryos. |
