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Fig. 5. Xdsh-D2 expression does not inhibit posterior neural cell fates. (A) Control embryo stained for HoxB9. HoxB9 staining is normal, extending from behind the head (arrow) to the tip of the tail (arrowhead). (B) Embryos injected animally at the eight-cell stage with 1 ng of Xdsh-D2; HoxB9 staining is present from the back of the head to the tip of the tail. (C) In embryos in which the posterior neural folds have completely failed to meet, two separate domains of HoxB9 expression can be seen. (c′) Anterior view of the embryo in C shows the two domains of HoxB9 expression, one on each side, extending from behind the head (arrows) to the posterior limit of each side of the embryo (arrowheads). (D) Control embryo stained for Krox20. (E) Krox-20 staining pattern is normal in embryos injected animally with Xdsh-D2 mRNA at the eight-cell stage. (F) In some severely affected embryos, Krox20 staining pattern is mildly distorted, but both stripes are discernible. (d′-f′) Detail of staining patterns for embryos in D-F; arrowhead marks stripe in rhombomere 3; arrows mark rhombomere 5. (G-H) Xdsh-D2 expression in the mesoderm does not inhibit posterior neural cell fates. (G) Control embryos stained for HoxB9 and Krox20. (H) Embryos injected with 1ng of Xdsh-D2 mRNA into the dorsal vegetal blastomeres at the eight-cell stage and stained for HoxB9 and Krox20. |
