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Fig. 1. Structure and expression pattern of XTsh1a. (A) Schematic representation of XTsh1a protein structure. Boxes representing unconventional Teashirt-type Zn finger motifs (Zn) are marked in dark gray, classical Zn finger motifs in light gray, the predicted homeodomain (HD) is indicated in white. (B) Real-time RT-PCR analysis of XTsh1a expression in embryonic stages. Samples were normalized to levels of ornithine decarboxylase (ODC). For better comparison, transcript amounts of the stages are shown relative to stage 6 which was set to 100%. (C–E) Spatiotemporal expression of XTsh1a in neurula stages. Dorsal view, anterior is right. (F) Transverse vibratome section corresponding to the dashed line indicated in panel D. (G, H) Double whole-mount in situ hybridization with XTsh1a probe (brown) in combination with Krox-20 (G, red) or En-2 (H, red) probes. Dorsoanterior view, anterior is down. (I–K) Expression of XTsh1a in tailbud embryos. (I) Transverse vibratome section at the hindbrain level (stage 26). (J, K) Lateral view, anterior is right. mhb; midbrain–hindbrain boundary, nc; notochord, ne; neuroectoderm, nt; neural tube, pn; pronephros, r3/r5; rhombomeres 3/5, dn; diencephalon, o; olfactory placode. |
