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Fig. 3. Effect of truncated Sek-1 on XKrox-20 gene expression in the Xenopus hindbrain. RNA encoding truncated Sek-1 was microinjected into 1 cell of 2 cell Xenopus embryos, so that one half of the embryo received injected RNA. The embryos were allowed to develop to various neurula stages and fixed. In situ hybridisation was then carried out to analyse the expression pattern of XKrox-20, a molecular marker of r3 and r5. Photographs were taken of the mounted hindbrain. (A) Uninjected half of stage 14 embryo. (B) Injected half of stage 14 embryo. (C) Stage 15 embryo; the injected half is on the left. (D-F) Stage 16 embryos, with injected RNA present in the left (D) or right (E,F) half. r, rhombomere. The arrowheads indicate XKrox-20-expressing cells in even-numbered rhombomeres. Scale bars, 50 mm. |
