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Fig. 11. mHoxb1 fails to rescue somitogenesis. Non-injected control (A) and embryos injected in the LHS with mHoxb1 mRNA (B: 100%, n=27), PG1 MOs (C: 81%, n=32) or a combination of PG1 MOs and mHoxb1 (D: 91% rescued, n=23), were analysed for the expression of MyoD (somites), Krox-20 (rhombomeres 3 and 5) and engrailed-2 (midbrain–hindbrain boundary) at early tailbud stage. Embryos are shown from a lateral view with anterior side to the left. The co-injection of mHoxb1 fails to rescue the somite, as shown by the continuous MyoD expression. Krox-20 expression shows that both PG1 MO and mHoxb1 overexpression is effective. |
