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FIG. 2. The normal expression patterns of the in situ markers are shown at the stages used in this screen (stage 21 (A) and stage 28 (D)). All embryos are shown with anterior facing left. (A) Anterodorsal views and (D) lateral views. (A) Nrp-1 staining of general neural tissue (eyes, brain, and spinal cord). (B) krox-20 and Hoxb9 were stained simultaneously. krox-20 stains the R3 and R5 rhombomeres
of the hindbrain (black arrow) as well as a band of migrating neural crest cells (black arrowhead). Hoxb9 stains the spinal cord (red arrowhead). (C) MyoD stains the somites of the paraxial mesoderm which will give rise to skeletal muscle. (D) Pax-8 stains the pronephric tubule and duct of the developing kidney, as well as the otic vesicle of the developing ear (black arrowhead). (E) Nkx-2.5 stains the
developing heart. (F) a-globin stains the developing ventral blood islands. |
