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Fig. 2. Triple depletion of Bmp2, Bmp4 and Bmp7 results in greater dorsalization of the embryo than any single or double BMP knockdown. Embryos were fixed at tailbud stage and expression domains of four markers (Otx2, Krox20, Myod and Sizzled) were compared with control siblings (n=15 per experimental set). (A-H) Lateral view, anterior to the left. (A′-H′) Ventral view, anterior to the top. (H′) Dorsal view, anterior to the top. (A-D′) Uninjected controls and single Bmp2, Bmp4 and Bmp7 knockdowns. (E-G′) Double BMP knockdowns (2/4, 2/7 and 4/7). Note the dorsalized phenotype with increased Otx2 expression, radial expression of Myod in the posterior, and ventral loss of Sizzled. (H-H′) Triple knockdown of Bmp2, Bmp4 and Bmp7 produces further dorsalization. Expression of Krox20 in rhombomere 5 was seen over the entire circumference of the embryo down to the tip of the tail (white arrowheads). |
