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Fig. 4. Ablation of the NP2 domain reduces the otic expression of Pax8 in stage 23/24 embryos. Otx2 (forebrain and eyes) and Krox20 (rhombomeres 3 and 5, arrowheads) (A, B) and Pax8 (C, D) otic expression (arrows) in control embryos at stage 23/24. (E–H) Unilateral ablation of NP2 domain results in reduced Pax8 expression on the manipulated side (G–H; arrows). Sibling embryos after NP2 domain removal (E, F) show reduced Krox20 expression in the hindbrain (residual rhombomere 5 expression can be detected in these embryos; arrowhead) without affecting Krox20 expression domain in the neural crest (nc). Dorsal (A, C, E, G) and lateral (B, D, F, H) views, anterior to left. |