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Fig. 4. 5-HT2B influences cranial NCC development in a cell-autonomous manner. (A) Scheme of the cranial NCC transplantation assay. (B,C) Lateral view of stage 30 transplanted and wild-type embryos, respectively. (B) GFP fluorescence in transplanted cranial NCCs. (C) Cranial NCCs visualized by the XDll4 probe in a wild-type embryo. (D,E) Horizontal vibratome sections of Xenopus tadpole. The red staining identifies transplanted NCCs. (E) Magnification of the second pharyngeal arch of a transplanted embryo stained for n-β-gal. (F) Flat-mount preparation of a stage 49 transplanted embryo double stained for cartilage (blue) and GFP immunoreactivity (brown). Skeletal elements derived from the transplanted cranial NCCs are visualized by the presence of GFP immunoreactivity. Note the reduced quadrate (red arrow) and the presence of ectopic cartilage (black arrow). |
