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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
bmp4
|
|
laevis
|
NF stage 13
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cement gland primordium
,
anterior neural fold
|
phox2a
|
|
laevis
|
NF stage 20
|
ventral
,
head
,
anterior
|
th
|
|
laevis
|
NF stage 20
|
neuroectoderm
,
nervous system
,
neuron
,
head
|
insm1
|
|
laevis
|
NF stage 20
|
nervous system
,
neural plate
,
anterior neural fold
,
neuron
,
head
,
[+]
|
|
|
Figure 2. Differentiation of anteroventral cells is regulated by BMP and Notch. Anterior (A-D,I,J,L,N,O), lateral (E-H), or dorsal (K,M,P) views of neurula to early tail bud embryos are shown. A,B: Stage 13 embryo stained with BMP4 alone (A) or double stained with Phox2a (arrowhead) and BMP4 (B). C,D: Embryos injected with tBR mRNA show a reduction of Phox2a and tyrosine hydroxylase (TH) expression in the anteroventral region. E-H: Induction of Phox2a and TH by Xash1 is reduced by coinjection of tBr. I: Delta1 is transiently expressed in the anteroventral cells (arrowhead). J,K: Embryos injected with Notch1 ICD or Su(H)Ank show a decrease of IA1 expression in the neural plate primary neurons and anteroventral cells (arrowheads). L,M: Embryos injected with Delta1stu or Su(H)DBM mRNA show an increase of IA1 expression in the neural plate primary neurons, trigeminal placodes, and anteroventral cells (arrowheads). |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hand2
|
|
laevis
|
NF stage 24
|
ectoderm
,
pharyngeal arch
,
ventral
,
neuron
,
anterior
|
tlx1
|
|
laevis
|
NF stage 24
|
ectoderm
,
ventral
,
trigeminal ganglion
,
neuron
,
anterior
|
nkx2-5
|
|
laevis
|
NF stage 22
|
stomodeal-hypophyseal primordium
,
endoderm
,
foregut
,
cardiac mesoderm
|
elavl3
|
|
laevis
|
NF stage 32
|
otic vesicle
,
hindbrain
,
neuron
,
neural tube
|
neurog1
|
|
laevis
|
NF stage 32
|
hindbrain
,
neuron
,
neural tube
|
phox2a
|
|
laevis
|
NF stage 24
|
ectoderm
,
mandibular crest
,
hyoid crest
,
branchial crest
,
cranial neural crest
,
[+]
|
dpysl3
|
|
laevis
|
NF stage 24
|
forebrain
,
midbrain
,
neuroectoderm
,
neuron
,
head
,
[+]
|
dpysl3
|
|
laevis
|
NF stage 32
|
otic vesicle
,
hindbrain
,
neuron
,
neural tube
|
th
|
|
laevis
|
NF stage 24
|
ventral
,
neuron
,
anterior
|
insm1
|
|
laevis
|
NF stage 24
|
ectoderm
,
forebrain
,
midbrain
,
hindbrain
,
cranial ganglion
,
[+]
|
insm1
|
|
laevis
|
NF stage 11
to
NF stage 13
|
neuroectoderm
,
trigeminal placode
,
neural plate
,
neuron
|
insm1
|
|
laevis
|
NF stage 27
to
NF stage 32
|
hindbrain
,
adrenal medulla
,
dorsal pancreatic bud
,
neuron
,
neural tube
|
insm1
|
|
laevis
|
NF stage 18
to
NF stage 22
|
nervous system
,
ventral
,
neuron
,
anterior
|
vgll4
|
|
laevis
|
NF stage 27
|
ectoderm
,
epidermis inner layer
|
celf3
|
|
laevis
|
NF stage 24
|
ectoderm
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
celf3
|
|
laevis
|
NF stage 24
|
ectoderm
,
brain
,
forebrain
,
hindbrain
,
neuroectoderm
,
[+]
|
b3gat1l
|
|
laevis
|
NF stage 27
|
ectoderm
,
ventral
,
neuron
,
anterior
|
krt12.4
|
|
laevis
|
NF stage 24
|
ectoderm
,
epidermis
,
epidermis outer layer
,
non-neural ectoderm
|
muc2l
|
|
laevis
|
NF stage 18
|
cement gland primordium
|
|
|
Figure 1. Developmental expression of IA1. A-P: Dorsal views (A,B), anterior views (C,D), ventral views (E,F) and lateral views (G-P) of embryos at the indicated stages. A-C,G: IA1 is expressed in the neural plate primary neurons and in scattered cells in the anteroventral region of embryos (arrowhead). D: Xash1 is expressed at stage 12.5-13 in the anteroventral cells (arrowhead). E: Late neurula embryo double stained with IA1 and CG probes. IA1 (dark blue) punctated staining (arrowhead) is detected in front of the cement gland (light blue). F: Early tail bud embryo double stained with IA1 and Nkx-2.5 showing that IA1-positive cells are found in the developing heart field. H-O: High magnification views of the head region of early tail bud embryos stained with the indicated probes. Like IA1, Myt1, Phox2, Hand2, Hox11L2, Etr-1, CRMP-4, and TH are expressed in the anteroventral cells (arrowheads). P: HNK-1 antibody staining in early tadpole. Staining in anteroventral cells (arrowheads) is indicated. Q: Two-color double in situ hybridization of IA1 (dark blue) and epidermal keratin (light blue). Note that IA1 expressing cells are found directly below the epidermal keratin expression layer. R: One-color double in situ hybridization of IA1 (dark punctated staining) and vestigial like-4 (light uniform staining in the deep layer of the ectoderm). Expression of IA1 can be seen in cells within the deep layer (arrowheads) of the ectoderm as well as in cells adhering to the underside of the deep layer (arrow). S: Transversal section of a tail bud stage embryo showing IA1 expression in the dorsal pancreas. T: Embryo in P bisected at the level of the hindbrain showing IA1 staining in the pancreas and in the adrenal medulla. U,Y: Transverse sections through the hindbrain region. Note that IA1 is transiently expressed in differentiating neurons of the neural tube and that IA1 transcripts, like that of Delta1, are located immediately adjacent to the Ngnr1-positive cells. ElrC and CRMP4 are found more peripherally. Tg, trigeminal ganglia; an, anterior neural plate; am, adrenal medulla; dl, deep layer of the ectoderm; dp, dorsal pancreas; fm, forebrain and midbrain neurons; sl, superficial layer of the ectoderm; ov, otic vesicle. |