|
Gene |
Clone |
Species |
Stages |
Anatomy |
dhrs3.L
|
|
laevis
|
NF stage 4 (8-cell)
|
animal
|
dhrs3.L
|
|
laevis
|
NF stage 10
|
upper blastopore lip
|
dhrs3.L
|
|
laevis
|
NF stage 11
|
marginal zone
,
dorsal marginal zone
,
involuted dorsal mesoderm
|
dhrs3.L
|
|
laevis
|
NF stage 13
|
neural plate
,
floor plate
,
pre-chordal neural plate border
|
dhrs3.L
|
|
laevis
|
NF stage 15
|
roof plate
,
chordal neural plate
,
notochord
,
intermediate mesoderm
|
dhrs3.L
|
|
laevis
|
NF stage 19
|
chordal neural plate
,
intermediate mesoderm
,
cardiac mesoderm
|
dhrs3.L
|
|
laevis
|
NF stage 29 and 30
|
forebrain
,
midbrain
,
cement gland
,
pronephric duct
,
post-anal gut
,
[+]
|
dhrs3.L
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
midbrain
,
spinal cord
,
cement gland
,
pronephric duct
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
rdh10
|
|
laevis
|
NF stage 11
|
dorsal marginal zone
|
rdh10
|
|
laevis
|
NF stage 13
|
chordal neural plate
|
rdh10
|
|
laevis
|
NF stage 19
|
intermediate mesoderm
,
lateral plate mesoderm
|
rdh10
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
presomitic mesoderm
,
pharyngeal arch
,
eye
,
lateral plate mesoderm
,
[+]
|
rdh10
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
lens
,
forebrain
,
cement gland
,
pronephric duct
,
[+]
|
|
|
Fig. 2. Spatial expression pattern of dhrs3. Whole mount in situ hybridization was employed to examine the spatial expression of dhrs3 at different developmental stages as indicated. (A) Lateral view of a four-cell stage embryo. (B-F Dorsal view of early stage embryos. dhrs3 transcripts were localized in the dorsal blastopore lip and the ridge of neural fold. The expression of rdh10 in stage 11, 13 and 19 are shown in C'D'F' for comparison. (G-I) Frontal view of stage 13, 15, 19 embryos, which showed that dhrs3 expression was absent in the head, but present in the posterior edge of the up-folding neural tube. (J-L) Lateral view of stage 13, 15, 19 embryos, showing dhrs3 expression in ventral bilateral regions (arrow). (M-N Lateral views of tailbud and tadpole stage embryos, showing the expression pattern of dhrs3 and rdh10. The anterior region of a stage 35 embryo showing dhrs3 expression was magnified in (N). dhrs3 expression was detected in the forebrain, midbrain, pharynx, spinal cord and pronephros (arrow). In lateral views (J-N), anterior is to the left. Abbreviations: fb, forebrain; mb, midbrain; p, pharynx; sc, spinal cord; pn, pronephros. |
|
Gene |
Clone |
Species |
Stages |
Anatomy |
dhrs3.L
|
|
laevis
|
NF stage 11
|
blastopore lip
,
involuted dorsal mesoderm
,
upper blastopore lip
,
axial mesoderm
|
dhrs3.L
|
|
laevis
|
NF stage 13
|
chordal neural plate
,
notochord
,
intermediate mesoderm
,
floor plate
,
pre-chordal neural plate border
|
dhrs3.L
|
|
laevis
|
NF stage 15
|
chordal neural plate
,
notochord
,
intermediate mesoderm
,
axial mesoderm
|
dhrs3.L
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
forebrain
,
spinal cord
,
cement gland
,
hypochord
,
[+]
|
Display additional annotations [+]
|
|
Fig. 3. Transverse and longitudinal sections of embryos showing dhrs3 expression. Stage 11 embryo (A) was sectioned showing the expression
domain in dorsal blastopore lip and axial mesoderm (B). (C-D Transverse section from a stage 13 embryo (C), indicating the expression in notochord
and neuroectoderm. The higher magnification of framed region (D) is shown in (D. (E-G Longitudinal and transverse sections from a stage 15 embryo
(E) indicated dhrs3 was expressed in notochord and neuroectoderm. (H-N) Transverse sections of a stage 35 embryo (H) illustrating the expression in
the midbrain, lens, otic vesicles, ventral pharynx, gut, pronephros, dorsal spinal cord and notochord. Expression pattern of rdh10 is shown in (IM.
Abbreviations: bc, blastocoel; bpl, blastopore lip; ne, neuroepithelium; pm, paraxial mesoderm; nc, notochord; mb, midbrain; ov, otic vesicle; p, pharynx;
pn, pronephros; sc, spinal cord. |
|
|
|
dhrs3 (dehydrogenase/reductase (SDR family) member 3) gene expression in Xenopus laevis embryos, NF stage 35, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |