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Display additional annotations [+]
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Fig. 5. Gastrula and neurula stage expression. In situ hybridization to gastrula and neurual stage Xenopus embryos with Endocut [darmin], Vito [slc5a8.1] , β3 [] and Fetuinish (fetub) antisense probes. The two left panels show whole mount in situ preparations to stage 11 gastrula embryos, from a vegetal view (extreme left) and a mid-sagital section, with dorsal/anterior to the left. The superficial endoderm of the blastopore lip (red arrow head) expresses Vito and β3 but not Endocut. The stage 15–18 embryos are bisected mid-sagitally and shown with anterior to the left and dorsal up. The stage 20 embryos are shown in cross section with dorsal up. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
c3
|
|
laevis
|
NF stage 22
to
NF stage 39
|
fin
,
brain
,
neural crest
,
endoderm
,
liver diverticulum
,
[+]
|
impdh1
|
|
laevis
|
NF stage 22
to
NF stage 39
|
endoderm
,
midgut
,
hindgut
|
ctbs
|
|
laevis
|
NF stage 22
to
NF stage 39
|
endoderm
,
midgut
,
hindgut
,
posterior
|
|
|
Fig. 3. Genes expressed in the Xenopus posterior endoderm from stages 22–39. Whole mount in situ hybridizations with antisense IMP2 [impdh1], Chito [ctbs], Endocut [darmin] and complement C3 [c3] probes. The thin black line indicates the position along the anterior-posterior axis where the cross sections on the right were taken. For the whole mount preparations anterior is to the right and in sections dorsal is up. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
khdrbs1
|
|
laevis
|
NF stage 42
|
midgut
,
hindgut
,
stomach
,
foregut
,
proctodeum
,
[+]
|
fetub
|
|
laevis
|
NF stage 42
|
liver
|
c3
|
|
laevis
|
NF stage 42
|
hindgut
,
intestine
,
liver
,
foregut
|
ventx2.2
|
|
laevis
|
NF stage 42
|
midgut
,
hindgut
,
stomach
,
foregut
,
second pancreatic bud
,
[+]
|
impdh1
|
|
laevis
|
NF stage 42
|
endoderm
,
midgut
,
hindgut
,
intestine
|
ctbs
|
|
laevis
|
NF stage 42
|
intestine
|
ndrg1
|
|
laevis
|
NF stage 42
|
midgut
,
stomach
,
proctodeum
,
second pancreatic bud
,
ventral pancreatic bud
|
|
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Fig. 6. Gene expression in organ buds of the stage 42 gut tube. The top panel show a schematic of an isolated stage 42 Xenopus gut tube indicating the lung buds (lu; blue), Liver bud (li; red), gall bladder bud (gb), pancreatic bud (p; gene) and intestine (in). The lower eight panels show whole mount in situ preparations of isolated stage 42 gut tubes with NDRG1 [ndrg1], Sam68 [khdrbs1], Fetuinish [fetub] , Vent-2 [ventx2.2], IMP2 [impdh1], Chito [ctbs] , Endocut [darmin] and C3 [c3] antisense probes. |